Aptamer Details

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Tr-8 (ID# 8036)

Aptamer Chemistry: RNA

Target: CD25

Antigen/Target Category: Protein

Affinity (Kd): 35.7 nM (reported value)

Binding Conditions/Buffer: HEPES-buffered saline, pH=7.5 with 2mmol/L CaCl2

Binding Temp: 37°C

Refolding Protocol: Transcribed RNA aptamer library was folded at 2 µM concentration in AIM-V (BSA) medium by step-wise incubation at 95°C for 5 minutes, 65°C for 10 minutes, followed by 37°C for 20minutes

Comments: Structure Unconfirmed

Sequence ():

Length: 51

Molecular Weight: 13869.47

Extinction Coefficient: 605800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Veeramani, Suresh,et al."An RNA aptamer-based biomarker platform demonstrates high soluble CD25 occupancy by IL2 in the serum of follicular lymphoma patients" American Association for Cancer Research 7, (2019) 1511-1522

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