Aptamer Details

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Aptamer Chemistry: DNA

Target: Hemagglutinin (HA) from Influenza A

Antigen/Target Category: Protein

Affinity (Kd): 1.5 nM (reported value)

Binding Conditions/Buffer: 20 mM Hepes, 150 mM NaCl, 2 mM KCl, 2 mM MgCl2, 2 mM CaCl2. 1 × washing buffer (pH 7.4)

Binding Temp: 23°C

Refolding Protocol: Prepare 1uM Aptamer in binding buffer, heat to 95°C for 10min, 4°C for 10min, 23°C for 5min

Comments: Kd of H5N1=1.5nM; Kd of H7N9=3.7nM; Kd of H9N2=10.1nM

Sequence ():

Length: 60

Molecular Weight: 18329.9 g/mole

Extinction Coefficient: 560500 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

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