Aptamer Details

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upanap-12 (ID# 7837)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA

Target: Urokinase-type plasminogen activator

Antigen/Target Category: Protein

Affinity (Kd): N/A nM (reported value)

Binding Conditions/Buffer: HBS, 2 mM MgCl₂, 0.1% BSA, and 0.025% Tween20

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Human urokinase-type plasminogen activator

Comments: Authors provided IC₅₀,values for inhibition of interaction between human uPA and uPAR with upanap-12 having the lowest value.

Sequence ():

Length: 79

Molecular Weight: 25480.22

Extinction Coefficient: 805500

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Daniel Miotto Dupont, Jeppe Buur Madsen, Roland Karl Hartmann, et al. Serum-stable RNA aptamers to urokinase-type plasminogen activator blocking receptor binding. RNA 2010 16: 2360-2369

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