Aptamer Details

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Vascular Permeability Factor/Vascular Endothelial Growth Factor (NX-213) (ID# 7547)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: Chimeric

Target: Vascular Permeability Factor/Vascular Endothelial Growth Factor (VPF/VEGF)

Antigen/Target Category: Protein

Affinity (Kd): 140 pM (reported value)

Binding Conditions/Buffer: Selection Buffer (PBS: 10.1 mM Na₂HPO₄, 1.8 mM KH₂PO4, 137 mM NaCl and 2.7 mM KCl (pH 7.4)) with 0.01 % human serum albumin

Binding Temp: 37°C

Refolding Protocol: Heated at 90°C for 2 min and then cooled on ice.

Specificity: Binding with Reduced VPF/VEGF, platelet-derived growth factor AB, basic fibroblast growth factor, antithrombin III, and thrombin was measured. Aptamer bound with >650- fold preference to target.

Comments: The sequence is denoted with d=deoxy, r=ribo, n=2'-NH2, m=2'-O-Me, s=phosphorothioate, and p=phosphate
Structure shown omits 5'- and 3'-terminal phosphorothioate thymine linkers.

Sequence ():

Length: 32

Molecular Weight: 16446.5

Extinction Coefficient: 300400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Green L, Jellinek D, Janjic N, et al. (1995) "Nuclease-resistant nucleic acid ligands to vascular permeability factor/vascular endothelial growth factor." Chem Biol. 2:683-695.

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