Aptamer Details

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VEGF (SL2-B aptamer) (ID# 7688)

Aptamer Chemistry: DNA

Target: VEGF (165)

Antigen/Target Category: Protein

Affinity (Kd): 0.5 nM (reported value)

Binding Conditions/Buffer: PBS and 0.005% tween-20 solution mixture was used as the running buffer, and 50 mM NaOH as the regeneration buffer.

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 26

Molecular Weight: 8034.25

Extinction Coefficient: 242600

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Kaur H, Yung L-YL (2012) Probing High Affinity Sequences of DNA Aptamer against VEGF165. PLoS ONE 7(2): e31196. doi:10.1371/journal.pone.0031196

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