Aptamer Details

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VRF-HS_B7H3-01 (Aptamer Against B7H3, Retinoblastoma, Weri-RB1) (ID# 8803)

Aptamer Chemistry: DNA

Target: B7H3

Antigen/Target Category: Protein

Affinity (Kd): 32.12 nM (reported value)

Binding Conditions/Buffer: 25 mM Glucose, 0.9 mM CaCl2, 5 mM MgCl2 in Dulbecco’s PBS without calcium and magnesium, 0.1 mg/ml yeast tRNA, 0.1 mg/ml Salmon sperm DNA, 5% FBS, and 1 mg/ml BSA.

Binding Temp: 4°C

Refolding Protocol: n/a

Specificity: Recognizes B7H3, Retinoblastoma lysate, Weri-RB1 cell lysate, WEri-RB1 cell secretome. Does not recognize BSA or anti-mouse IgG HRP conjugated secondary antibody.

Sequence ():

Length: 86

Molecular Weight: 26668.3

Extinction Coefficient: 857400 L/(mole*cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Maradani, B.S., Parameswaran, S. & Subramanian, K. Development of DNA aptamers targeting B7H3 by hybrid-SELEX: an alternative to antibodies for immuno-assays. Sci Rep 14, 13552 (2024). https://doi.org/10.1038/s41598-024-64559-7

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