Aptamer Details

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YPEN-1 Endothelial Cells (III.1) (ID# 7605)

Aptamer Chemistry: DNA

Target: YPEN-1 Endothelial Cells

Antigen/Target Category: Cells

Affinity (Kd): N/A nM (reported value)

Binding Conditions/Buffer: Selection buffer: 50 mM Tris-HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl₂, and 0.1% NaN3 with 1 ug/uL yeast tRNA.

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 96

Molecular Weight: 29854.53

Extinction Coefficient: 961400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Blank et al. "Systematic Evolution of a DNA Aptamer Binding to Rat Brain Tumor Microvessels." The Journal of Biological Chemistry, >b>276(2001): 16464-16468.

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