Aptagen, LLC
RNA
Hen Egg White Lysozyme
Protein
2.5 µM (reported value)
Ligation buffer (50 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2)
25°C
Denatured at 70° C for 3 minutes in 5 uL of water. Then, allowed to cool to RT.
Contains a L1 ligase ribozyme motif.
Hen Egg White Lysozyme (cyt7-2) (ID# 259)
5'rGprGprAp rCprCprUp rCprGprGp rCprGprAp rAprAprGp rCp rUprAprAp rCprGprUp rCprUprCp rAprUprGp rGprCprUp rAprAprAp rUprUprGp rCprCprAp rUprGprUp rUprGprCp rUprAprCp rAprAprAp rUprGprAp rUprAprUp rGprAprCp rUprAprGp rAp rGprAprGp rGprUprUp rAprGprGp rUprGprCp rCprUprCp rGprUprGp rAprUprGp rUprCprCp rAprGprUp rCprGprCp 3'
106
30571.4 g/mole
942500 L/(mole·cm)
49.47%
1.06
32.44
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Robertson and Ellington. "In vitro selection of nucleoprotein enzymes." Nature Biotechnology, 19(2001): 650-655. Hesselberth et al. "Simultaneous detection of diverse analytes with an aptazyme ligase array." Analytical Biochemistry, 312(2003): 106-112.
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