Aptagen, LLC
DNA
C-Reactive Protein (CRP)
Protein
3.51 nM (reported value)
DNA and CRP-conjugated magnetic beads mixed and incubated utilizing a membrane-type micropump/micromixer (for about 5min). Dissociation constant determined using a SPR technique. SPR Buffer: 10 mM Tris buffer with 50 mM NaCl and 2 mM CaCl2.
25°C
Not mentioned in the reference.
C-Reactive Protein (CRP) (ID# 353)
5'dGpdGpdCp dApdGpdGp dApdApdGp dApdCpdAp dApdAp dCpdApdCp dGpdApdTp dGpdGpdGp dGpdGpdGp dGpdTpdAp dTpdGpdAp dTpdTpdTp dGpdApdTp dGpdTpdGp dGpdTpdTp dGpdTpdTp dGpdCpdAp dTpdGpdAp dTpdCpdGp dTpdGpdGp dTpdCpdTp dGpdTpdGp dGpdTpdGp dCpdTpdGp dTp3'
80
22571.71 g/mole
704600 L/(mole·cm)
51.39%
1.42
32.03
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
C-J Huang et al. "Integrated microfluidic system for rapid screening of CRP aptamers utilizing systematic evolution of ligands by exponential enrichment (SELEX)." Biosens. Bioelectron. 25(2010):1761-1766.
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