Aptagen, LLC
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
2'-F-RNA
EGFR - Extracellular Domain
Protein
10 nM (reported value)
Buffer used for Binding Assays: PBS with 0.01% BSA. Incubation Time: 15 minutes.
37°C
85°C for 5 minutes in DMEM serum free, snap-cooled on ice for 2 minutes, gradual warming to 37°C (from article reference [34])
High affinity for human extracellular EGFR (both disulfide-linked EGFR dimer and reduced EGFR monomer). No appreciable binding to extracellular ErbB3.
This apatamer was shown to induce apoptosis in A549 cells and inhibit tumor growth in human non-small-cell lung cancer xenograft mice.
EGFR (CL4) (ID# 446)
5'rGpfCpfCpfUpfUprAprGpfUprAprApfCprGpfUprGpfCpfUpfUpfUprGprApfUprGpfUpfCprGprApfUpfUpfCprGprApfCprAprGprGprAprGprGpfCp3'
39
12561.44 g/mole
386200 L/(mole·cm)
51.28%
2.59
32.53
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
CL Esposito et al. A neutralizing RNA aptamer against EGFR causes selective apoptotic cell death. PLoS One 6(2011):e24071.
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