Aptagen, LLC
RNA
Sialyl Lewis X
Other
0.085 nM (reported value)
Binding Buffer (150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2)
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
The reported sequence contains Y(pyrimidine) at site 46 and 67. We've chosen uracil (U) to replace the arbitrary Y. The reported sequence contains R(purine) at site 101. We've chosen adenine(A) to replace the arbitrary R.
Sialyl Lewis X (5) (ID# 48)
5'rCprCprGprUprAprAprUprAprCprGprAprCprUprCprAprCprUprAprUprAprGprGprGprGprAprGprCprUprCprGprGprUprAprCprCprGprAprAprUprUprCprAprAprGprGprUprAprCprUprCprUprGprUprGprCprUprUprGprUprCprGprAprUprGprUprGprUprAprUprUprGprAprUprGprGprCprAprCprUprUprUprCprGprAprGprUprCprAprAprCprGprAprGprUprUprGprAprCprAprGprAprAprCprAprAprGprUprAprGprUprCprAprAprGprCprUprUprUprGprCprAprGprAprGprAprGprGprAprUprCprCprUprUp3'
133
42804.72 g/mole
1336400 L/(mole·cm)
46.62%
0.75
32.03
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Jeong, Sunjoo, et al. "In Vitro Selection of the RNA Aptamer against the Sialyl Lewis X and Its Inhibition of the Cell Adhesion." Biochemical and Biophysical Research Communications, 281 (2001): 237ƒ??243.
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