Aptamer Details
- Aptamer Chemistry
- RNA
- Target
- Sialyl Lewis X
- Antigen/Target Category
- Other
- Affinity (Kd)
- 0.085 nM (reported value)
- Binding Conditions/Buffer
- Binding Buffer (150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2)
- Binding Temp
- RT
- Refolding Protocol
-
NA
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
- Comments
- The reported sequence contains Y(pyrimidine) at site 46 and 67. We've chosen uracil (U) to replace the arbitrary Y. The reported sequence contains R(purine) at site 101. We've chosen adenine(A) to replace the arbitrary R.
- Sequence ( )
-
5'--3'
- Length
- 133
- Molecular Weight
- 42804.72 g/mole
- Extinction Coefficient
- 1336400 L/(mole·cm)
- Reference
-
Jeong, Sunjoo, et al. "In Vitro Selection of the RNA Aptamer against the Sialyl Lewis X and Its Inhibition of the Cell Adhesion." Biochemical and Biophysical Research Communications, 281 (2001): 237ƒ??243.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Related Aptamer Targets
There are no aptamers related to this aptamer.
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